Abstract—In vitro Moringa oleifera Lam. seedlings were successfully produced through seed culture on Murashige & Skoog (MS) agar medium containing 3% (w/v) sucrose and 0.2% (w/v) GelriteTM in the absence of growth regulators under 1,500 lux of light density, 16 hour photoperiod light at temperature of 25 ± 2°C. Shoot-derived callus and root-derived callus of M. oleifera were established via culture of shoot and root on the MS medium supplemented with 0.5 mg/l of 2,4-dichlorophenoxy acetic acid (2,4-D) in the dark at 25 ± 2°C. Stem, leaf and root of native M. oleifera and M. oleifera callus were assayed for peroxidase activity using guaiacol as a substrate of the enzyme. In native plant, crude extract from root provided the highest peroxidase specific activity, followed by those from stem and leaf with the specific activity of 19.73 ± 0.18, 16.56 ± 1.43 and 13.38 ± 1.04 unit/mg protein, respectively. Crude extract of root-derived callus and shoot-derived callus of M. oleifera possessed specific activity of 167.25 ± 16.12 and 103.99 ± 10.64 unit/mg protein. These values are significantly higher than their counter parts from native M. oleifera suggesting the potential use of the callus cultures as new and improved sources of peroxidase.