Abstract—Precision electronics which can provide multi-channel stimulation capability is important part for neurorobotic and investigation of neuronal development and plasticity. Towards this end, an electrical stimulator designed for dissociated neurons on multi-electrode arrays (MEA) is present in this paper. The stimulator is mainly designed for online application in systems requiring simulation stimulation and recording signal from dissociated neurons on MEAs. The developed stimulator includes 64 independent channels, which are able to generate the arbitrary defined biphasic voltage waveform, controlled in real time with time resolution of 3us and amplitude resolution of 12 bits. The stimulator can generate three basic waveforms (rectangle, sine and triangle wave) in default. The amplitude of the output signal produced by this stimulator can achieve a very wide range. The preliminary experiments in which neuronal activities are evoked by this stimulator have been done. Moreover, the additional analysis of neuronal activities evoked by this stimulator will