Abstract—When working with cells, the first step is often to lyse them in order to get the cellular content before analysis. Some bacteria, especially gram-positive, may be difficult to lyse with conventional lysis buffer. If the cells are not properly lysed, the quality of the analysis results might suffer. In this paper, a planar micromixer design based on a splitting and recombination (SAR) concept with ellipse-like micropillars is proposed for cell lysis. With a splitting and recombination concept, the diffusion distance of the fluids in a micromixer is decreased. Thus, space usage for micromixer of an automatic sample collection system is also minimized. The efficiency of the proposed micromixer is comparable to other conventional lysis methods.

Index Terms—micromixer, passive mixing, splitting and recombination, microfabrication, lysis

Cite: Nhut Tran-Minh, Birgitte Kasin Hønsvall, and Frank Karlsen, "Integrated Microfluidic Device for Cell Lysis in a Continuous Flow Mode," Journal of Medical and Bioengineering, Vol. 4, No. 2, pp. 150-153, April 2015. Doi: 10.12720/jomb.4.2.150-153