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Dual Carbon Fermentation for the Production of Inducible Cellobiohydrolase by Recombinant Aspergillus Niger

Norashikin Othman1, Jamaliah Md. Jahim1, Abd. Munir Abd. Murad2 , and Farah Diba Abu Bakar2
1.Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia
2.School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia
Abstract—This study investigates the use of two carbon sources in fermentation for the production of cellobiohydrolase (CBH), an adaptive or inducible enzyme. Selection of carbon source was first done using single substrate cultivation between glucose, maltose and lactose using recombinant A. niger PY11. This recombinant microorganism will produce CBH in high quantity, with maltose acting as its main inducer. Glucose and maltose were selected in dual carbon fermentation, as glucose produce highest biomass 0.32g/ml at day 4th and maltose produce 0.42g/ml biomass at day 9th. Cultivation of A. niger was done for dual carbon containing sugar glucose and maltose with concentration ratio 1:1. Cultivation with medium containing maltose was done for comparison between single carbon and dual carbon. For dual carbon cultivation, highest biomass form at day 7th at 0.44g/ml while specific enzyme activity reached 10.54 U/g at day 8th. For single substrate, highest biomass production was recorded at day 9th with 0.41g/ml.while specific enzyme activity at day 7th at 4.02 U/g.

Index Terms—cellobiohydrolase (CBH), dual carbon cultivation, recombinant Aspergillus niger. static
surface liquid culture. (SSCL)

Cite: Norashikin Othman, Jamaliah Md. Jahim, Abd. Munir Abd. Murad, and Farah Diba Abu Bakar, "Dual Carbon Fermentation for the Production of Inducible Cellobiohydrolase by Recombinant Aspergillus Niger," Journal of Medical and Bioengineering, Vol. 3, No. 2, pp. 93-97, June 2014. Doi: 10.12720/jomb.3.2.93-97
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