Abstract—A laser sensor based on Laser Induced Breakdown Spectroscopy (LIBS) was developed for analysis of human gallbladder stones specifically for determination of heavy metals. In addition to qualitative analysis, the concentrations of the heavy metals were determined in different gallbladder stones samples using LIBS technique for the spectral lines of Cr I at 425.4 nm, Pb I at 368.3 nm, Cd I at 291.5, Ni at I 361.9 nm and Hg I at 366.3 respectively. The limit of detection of our LIBS system was determined as well for these five elements. The concentrations of above mentioned elements detected with Our LIBS system were in good agreement with ICP-results. In addition, the plasma parameters like temperature and electron density were also estimated using the so called Boltzmann plot and Boltzmann distribution of upper energy levels and Stark broadening, respectively. The plasma temperature and electron density data for gallbladder stones was essential to testify that the laser induced plasma fulfils Local Thermal Equilibrium condition which is highly critical for the quantitative LIBS analysis.

Index Terms—laser technology and its applications, laser induced breakdown spectroscopy, gallbladder stones analysis, toxic metals

Cite: Mohammed A. Gondal, Mohamed A. Shemis, Bilal Gondal, and Ahmed A. I. Khalil, "Gallbladder Stones Analysis Using Pulsed UV Laser Induced Breakdown Spectroscopy," Journal of Medical and Bioengineering, Vol. 5, No. 2, pp. 85-88, April 2016. Doi: 10.18178/jomb.5.2.85-88