General Principles of Real-Time PCR: A Technology for Quantitative Detection of Phytopathogens
Seyed Mahyar Mirmajlessi 1, Evelin Loit 1, Marika Mänd 2
1. Department of Field Crops and Grassland Husbandry, Estonian University of Life Sciences, Tartu, Estonia
2. Department of Plant Protection, Estonian University of Life Sciences, Tartu, Estonia
2. Department of Plant Protection, Estonian University of Life Sciences, Tartu, Estonia
Abstract—Real-time PCR provides a rapid and specific method for detection of plant pathogens in various environmental samples. The technique incorporates traditional PCR efficiency with the production of a specific fluorescent signal, providing quantification of specific targets. There are four main chemistries, which can be clustered into the non-specific DNA-binding fluorophores and the specific fluorophore-labelled oligonucleotide probes. In the present review, we describe the general background of major real-time chemistries as well as some considerations for assay development. Basically, Scorpion and SYBR green have the most and least sensitivities between available chemistries, respectively. However, with accurate optimization, real-time PCR can provide reliable and high throughput detection of target DNA in various environments.
Index Terms—alternative chemistries, plant pathology, real-time PCR
Cite: Seyed Mahyar Mirmajlessi, Evelin Loit, and Marika Mänd, "General Principles of Real-Time PCR: A Technology for Quantitative Detection of Phytopathogens," Journal of Medical and Bioengineering, Vol. 5, No. 1, pp. 49-52, February 2016. Doi: 10.12720/jomb.5.1.49-52
Index Terms—alternative chemistries, plant pathology, real-time PCR
Cite: Seyed Mahyar Mirmajlessi, Evelin Loit, and Marika Mänd, "General Principles of Real-Time PCR: A Technology for Quantitative Detection of Phytopathogens," Journal of Medical and Bioengineering, Vol. 5, No. 1, pp. 49-52, February 2016. Doi: 10.12720/jomb.5.1.49-52
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