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Production and Characterization of Xylanases from Fungal Isolate Aspergillus Terreus and Development of Low Cost Fermentation Media

Prachi Kaushik and Anushree Malik
Applied Microbiology Laboratory, Centre for Rural Development and Technology, Indian Institute of Technology Delhi, New Delhi, India
Abstract—Fungal isolate, Aspergillus terreus, was utilized for the production of hydrolytic enzymes through solid state fermentation of wheat bran. After 5 days of incubation, 116 U/g xylanase, 1.53 fpu/g cellulase and 16.99 cmc/g carboxymethyl cellulase was produced. The optimum pH and temperature for the activity of xylanase enzyme was found to be pH 5 and 60ºC. The zymogram analysis of the native enzyme shows the presence of single xylanase band with the molecular weight of 91.67 KDa. Low cost nutrient supplements such as the corn cob as the carbon substrate and biogas slurry as the nitrogen supplement can offer a low cost medium for the production of xylanase enzyme by A. terreus.

Index Terms—agricultural residue, biogas slurry, characterization, fungi, xylanase

Cite: Prachi Kaushik and Anushree Malik, "Production and Characterization of Xylanases from Fungal Isolate Aspergillus Terreus and Development of Low Cost Fermentation Media," Journal of Medical and Bioengineering, Vol. 5, No. 1, pp. 15-18, February 2016. Doi: 10.12720/jomb.5.1.15-18
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