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Whole-Body Imaging of Bacteria Expressing mKate2 Fluorescence

Ivan Vuletic1,2, Alison Ren1, Jiaxuan Li1 , Yichen Ding1, and Jun Li2
1.Department of Biomedical Engineering/College of Engineering, Peking University, No. 5 Yiheyuan Road, Beijing 100871, China
2.Laboratory Animal Center/Peking University, No. 5 Yiheyuan Road, Beijing 100871, China
Abstract—We established and validated a non-pathogenic bacteria to express a far red fluorescence protein mKate2 for in vivo studies in mice. Using the fluorescence reflectance imaging (FRI) system, the bacteria expressing mKate2 was tested non-invasively and in real-time in different mouse body compartments, including subcutaneous, abdominal, and gastrointestinal tract. Our results suggested that bacteria colonization could be clearly visible and successfully monitored over time in live animals. This method could be a prospective approach for further studying of either pathogenic or non-pathogenic bacterial infection, antibiotic therapy or bacteria as drug-gene delivery for tumor therapy in small animal model such as mice.

Index Terms—bacteria imaging, mKate2, fluorescence

Cite: Ivan Vuletic, Alison Ren, Jiaxuan Li, Yichen Ding, and Jun Li, "Whole-Body Imaging of Bacteria Expressing mKate2 Fluorescence," Journal of Medical and Bioengineering, Vol. 4, No. 5, pp. 381-385, October 2015. Doi: 10.12720/jomb.4.5.381-385
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