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Vasa Vasorum Angiogenesis through Increased Levels of H2O2, HIF-1α, NF-κB and INOS: In Vivo Study of Atherosclerosis

Titin A. Wihastuti1, Djanggan Sargowo2, Setyawati Soeharto3 , Teuku Heriansyah4, and Grace R. Widyasih5
1.Department of Biomedical, Medical Faculty, Brawijaya University, Malang, Indonesia
2.Department of Cardiology, Faculty of Medicine, Brawijaya University, Malang, Indonesia
3.Departement of Pharmacology, Faculty of Medicine, Brawijaya University, Malang, Indonesia
4.Department of Cardiology, Faculty of Medicine, Syiah Kuala University, Aceh, Indonesia
4.Department of Medical Education Programs, Faculty of Medicine, Brawijaya University, Malang, Indonesia
Abstract—This study aims to determine the increased of vasa vasorum angiogenesis through H2O2, HIF-1α, NFkB and iNOS pathways in Wistar strain of Rattus novergicus given HFD. This research is a true experimental laboratory, with in vivo approach to post-test with control group, using 24 males wistar strain of Rattus novergicus, 8 weeks of age, were divided into 6 groups. Those animal model was given HFD serially for 1, 3 and 5 months. Each series contained a control group with normal diet (ND). The parameter in this study are H2O2 measured by colorymetric; NFkB, iNOS and HIF-1α were measured using double staining imunofluoresence which was observed with CLSM (Confocal Lasser Scaning Microscope) in aortic smooth muscle cells; vasavasorum angiogenesis were measured from VEGFR1 levels in aortic tissue, and confirmed by HE staining for anatomical and histopathological verification. The results of ANOVA analysis, path analysis and Pearson correlations showed that HFD affect the vasa vasorum angiogenesis through H2O2, NFkB and iNOS in wistar strain of Rattus novergicus (p < 0.05). The data show that NFkB plays greater role in regulating inflammation and affect vasa vasorum angiogenesis, starting at 3 months of HFD administration. It is known from the role of iNOS on levels of VEGFR–1 that is greater than the role of HIF-1α. The conclusion of this study is the vasa vasorum angiogenesis is more dominantly influenced by inflammatory conditions. 

Index Terms—vasa vasorum angiogenesis, H2O2, HIF-1α, NF-kB, iNOS, atherosclerosis, hypercholesterol diet.

Cite: Titin A. Wihastuti, Djanggan Sargowo, Setyawati Soeharto, Teuku Heriansyah, and Grace R. Widyasih, "Vasa Vasorum Angiogenesis through Increased Levels of H2O2, HIF-1α, NF-κB and INOS: In Vivo Study of Atherosclerosis," Journal of Medical and Bioengineering, Vol. 4, No. 5, pp. 342-349, October 2015. Doi: 10.12720/jomb.4.5.342-349
 
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