Immobilization of Glucose Oxidase on Gold Surface for Applications in Implantable Biosensors
Kuo-Yuan Hwa1and Boopathi Subramani2
1.Department of Molecular Science and Engineering, Institute of Organic and Polymeric Materials, Center for Biomedical Industry, National Taipei University of Technology, Taipei, Taiwan, ROC
2.Department of Molecular Science and Engineering, National Taipei University of Technology, Taipei, Taiwan, ROC
2.Department of Molecular Science and Engineering, National Taipei University of Technology, Taipei, Taiwan, ROC
Abstract—Surface modification is an important step in developing nanoscale biosensors. The uniform and stable surface structure by forming self-assembled monolayers (SAM) makes it suitable for the development of biosensors. We have studied several surface modification methods on a gold electrode to covalently immobilize the glucose oxidase. The methods with SAM formation were more efficient for immobilizing glucose oxidase, as expected. Furthermore, the gold surface modified with MPA and the coupling reagents EDC and NHS was found to be the best method among the various matrices tested. With highly efficient modified matrix, we hope to develop a continuous glucose monitoring system in the future.
Index Terms—microneedle, glucose oxidase, subcutaneous tissue, continuous glucose monitoring system
Cite: Kuo-Yuan Hwa and Boopathi Subramani, "Immobilization of Glucose Oxidase on Gold Surface for Applications in Implantable Biosensors," Journal of Medical and Bioengineering, Vol. 4, No. 4, pp. 297-301, August 2015. Doi: 10.12720/jomb.4.4.297-301
Index Terms—microneedle, glucose oxidase, subcutaneous tissue, continuous glucose monitoring system
Cite: Kuo-Yuan Hwa and Boopathi Subramani, "Immobilization of Glucose Oxidase on Gold Surface for Applications in Implantable Biosensors," Journal of Medical and Bioengineering, Vol. 4, No. 4, pp. 297-301, August 2015. Doi: 10.12720/jomb.4.4.297-301
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