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The Effects of Novel AMPK Activator on Human Vascular Endothelial Cells

Han-Min Chen1,2, Jiun-Tsai Lin3, Cheng-Yi Kuo3 , and Chun-Fang Huang3
1.Catholic Fu-Jen University/Department of Life Science, New Taipei City, Taiwan
2.Catholic Fu-Jen University/Institute of Applied Science and Engineering, New Taipei City, Taiwan
3.Energenesis Biomedical Co. Ltd., New Taipei City, Taiwan
Abstract—Adenosine 5’-monophosphate-activated protein kinase (AMPK) is a key regulator of cellular energy homeostasis and involved in modulating several important cellular mechanism including inflammation. Here we demonstrated that a novel AMPK activator, ENERGI-F704, dose-dependently activated AMPK in human umbilical vein endothelial cells (HUVECs). The pharmacological AMPK inhibitor, compound C, abolished the phosphorylation of threonine 172 residue on AMPK induced by ENERGI-F704. Importantly, ENERGI-F704 treatment did not affect HUVECs viability at the tested concentration. It was also observed that ENERGI-F704 significantly reduced the expression of inflammation cytokine, IL-6, in the high glucose cultured HUVECs during the long culture period. Furthermore, ENERGI-F704 suppressed the high glucose induced monocytotic adhesion to HUVECs. Collectively, our data demonstrated that ENERGI-F704 is of use in the application of attenuating the high glucose induced chronic inflammation in endothelial cells.

Index Terms—AMPK, monocyte adhesion, AMPK activator

Cite: Han-Min Chen, Jiun-Tsai Lin, Cheng-Yi Kuo, and Chun-Fang Huang, "The Effects of Novel AMPK Activator on Human Vascular Endothelial Cells," Journal of Medical and Bioengineering, Vol. 3, No. 2, pp. 144-148, June 2014. Doi: 10.12720/jomb.3.2.144-148
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