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Proliferative Capacity of in Vitro Corneal Epithelium: Role of Acacia Honey in the Initial Step of Wound Healing

Choy Ker-Woon1, Norzana Abd Ghafar1, Chua Kien Hui2 , Yasmin Anum Mohd Yusof3, and Ng Sook Luan2
1.Department of Anatomy, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia
2.Department of Physiology, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia
3.Department of Biochemistry, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia
Abstract—Proliferation of corneal epithelial cells (CEC) isvital in the initial stage of wound healing. This study aimed to investigate the proliferative capacity of Acacia Honey (AH) on rabbit CEC via assessment on morphology, proliferation, cell cycle, gene and protein expressions.The optimal dose of AH in basal medium (BM) and complete cornea medium (CCM) was identified via MTT assay. CEC cultured in both media supplemented with 0.025% AH showed optimal proliferative capacity compared to the control. There were no abnormal changes in morphology and cell cycle analysis. Gene and protein expression of CK3 was increased in the CEC cultured with 0.025% AH in both media. CEC cultured in media supplemented with 0.025% AH promotes proliferation while retaining its normal morphology, cell cycle, gene and protein expressions. These promising results serve as an impetus in realizing the proliferative potential of AH in promoting the initial step of corneal wound healing.

Index Terms—Acacia Honey, proliferation, corneal epithelial cells, corneal wound healing.

Cite: Choy Ker-Woon, Norzana Abd Ghafar, Chua Kien Hui, Yasmin Anum Mohd Yusof, and Ng Sook Luan, "Proliferative Capacity of in Vitro Corneal Epithelium: Role of Acacia Honey in the Initial Step of Wound Healing," Journal of Medical and Bioengineering, Vol. 3, No. 2, pp. 107-112, June 2014. Doi: 10.12720/jomb.3.2.107-112
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