MAPKs Modulate RPE Response to Oxidative Stress
Zhaohui Wang1, Thomas J. Bartosh2, Min Ding3
, and Rouel S. Roque4
1.Gradalis, Inc., 1700 Pacific Ave., Suite 1100, Dallas, Texas, 75201, USA
2.Texas A&M HSC, Institute for Regenerative Medicine at Scott & White Hospital, Temple, TX 76502 USA
3.Dept. of Integrative Physiology, University of North Texas HSC, Fort Worth, Texas 76107 USA
4.Dept. of Basic Sciences, Touro University Nevada, Henderson NV 89014 USA
2.Texas A&M HSC, Institute for Regenerative Medicine at Scott & White Hospital, Temple, TX 76502 USA
3.Dept. of Integrative Physiology, University of North Texas HSC, Fort Worth, Texas 76107 USA
4.Dept. of Basic Sciences, Touro University Nevada, Henderson NV 89014 USA
Abstract—To investigate the role of mitogen-activatedprotein kinases (MAPK) in the response of the retinal pigment epithelium (RPE) to oxidative stress (OS), a well-characterized RPE cell line (ARPE-19) was exposed to an oxidant-generating system catalyzed by glucose oxidase and glucose (GO/G). ARPE-19 cells were characterized for morphological changes, mitochondrial membrane permeability (MMP), and cell survival following exposure to GO/G. The effects of GO/G on MAPK activity were determined by assaying for p38MAPK expression and activity in the presence or absence of SB203580, a specific p38MAPK inhibitors, or p38MAPK siRNA. ARPE-19 cells exposed to GO/G showed morphological changes, increased MMP, and cell death. Exposure to OS promoted increased phosphorylation of p38MAPK and hsp27, a downstream target of p38MAPK. SB203580, but not p38 MAPK siRNA, inhibited ARPE-19 cell death. In conclusion, activation of p38MAPK may promote downstream pathways responsible for the morphological changes observed in RPE cells during oxidative damaging, however, these pathways do not appear to be responsible for OS-induced RPE degeneration.
Index Terms—oxidative stress, retinal pigment epithelium, mitogen activated protein kinases, p38MAPK, age-related macular degeneration, reactive oxygen species
Cite: Zhaohui Wang, Thomas J. Bartosh, Min Ding, and Rouel S. Roque, "MAPKs Modulate RPE Response to Oxidative Stress", Journal of Medical and Bioengineering, Vol. 3, No. 1, pp. 67-73, March 2014. Doi: 10.12720/jomb.3.1.67-73
Index Terms—oxidative stress, retinal pigment epithelium, mitogen activated protein kinases, p38MAPK, age-related macular degeneration, reactive oxygen species
Cite: Zhaohui Wang, Thomas J. Bartosh, Min Ding, and Rouel S. Roque, "MAPKs Modulate RPE Response to Oxidative Stress", Journal of Medical and Bioengineering, Vol. 3, No. 1, pp. 67-73, March 2014. Doi: 10.12720/jomb.3.1.67-73
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