Abstract—Poly aromatic hydrocarbons (PAHS) and total petroleum hydrocarbon (TPH) on receiving water were a major concern in many country of the world specially producer of oil and related petrochemical products. Such pollutants often had an effect on aquatic environment and on human health. The removal of PAHS and TPH from one of Iran’s petrochemical effluent by using of a Bio-Enzyme mixture had been studied at laboratory scale. The investigation review Biochemical characterization of the sample in contact with application of bio-enzyme, by installing of batch reactor (with and without application said bio-enzyme). The batch reactor runs for 10 days and result shown that without presence of said bio-enzyme PAHS, TPH, and chemical oxygen demand (COD) did not remove and there is not any changes after 10 days , even the batch reactor by apply of a mixture of bio-enzyme removed TPH /PAH, and COD about 99.98%, and 100% after 10 days, respectively.Main factors that manage the removal process were the bio-enzyme activity in hydrolysis and oxidation of pollutants. As a result, the Bio-enzymes activity and the mineralization of pollutants due to presence of millions of net micro-Bio-enzymes were responsible for the removal TPH/PAH/COD.

Index Terms—Bio-enzyme, chemical oxygen demand, poly aromatic hydrocarbons (PAH), total petroleum hydrocarbon, (TPH).

Cite: M. Najafi and G. Kashi, "Removal of Poly Aromatic Hydrocarbons and Total Petroleum Hydrocarbon by Using of Bio-Enzyme: a Batch Study", Journal of Medical and Bioengineering vol. 1, no. 1, pp. 42-47, 2012. doi: 10.12720/jomb.1.1.42-47